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Dose of 3‐methylcholanthrene enhances vitamin C accumulation and mRNA expression of its transporter in the liver of ODS rats and in HepG2 cells
Author(s) -
Sone Yasuko,
Ueta Etsuko,
Sannomaru Yasuko,
Miyake Noriko,
Sone Hirohito,
Otsuka Yuzuru,
Kondo Kazuo,
Kurata Tadao,
Suzuki Emiko
Publication year - 2011
Publication title -
journal of biochemical and molecular toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.526
H-Index - 58
eISSN - 1099-0461
pISSN - 1095-6670
DOI - 10.1002/jbt.20403
Subject(s) - methylcholanthrene , ascorbic acid , vitamin c , vitamin , chemistry , transporter , biochemistry , endocrinology , medicine , carcinogen , biology , food science , gene
Polycyclic aromatic hydrocarbon (PAH) compounds including 3‐methylcholanthrene induce harmful reactive intermediates and reactive oxygen species. This study reports the effect of 3‐methylcholanthrene on the accumulation of vitamin C and the expression of vitamin C transporters. ODS rats were given l ‐ascorbic acid daily and intraperitoneal injections of 10 mg 3‐methylcholanthrene in total. On day 10, vitamin C concentrations and the expression of vitamin C transporter in the tissues were measured. As a result, the levels of sodium‐dependent vitamin C transporter (SVCTs) 1 and the l ‐ascorbic acid concentration in 3‐methylcholanthrene‐treated livers and hepatocytes have increased significantly. However, the content of vitamin C in the urine and TBARS in the liver have not changed. These results suggest that the administration of 3‐methylcholanthrene elevates the requirement for vitamin C via (SVCTs) 1 due to xenobitics‐metabolizing, such as the induction of cytochrome P450 family. © 2011 Wiley Periodicals, Inc. J Biochem Mol Toxicol 25:369–376 2011; View this article online at wileyonlinelibrary.com . DOI 10.1002/jbt.20403