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Trans fat diet causes decreased brood size and shortened lifespan in Caenorhabditis elegans delta ‐6‐desaturase mutant fat ‐3
Author(s) -
Reisner Kaja,
Lehtonen Marko,
Storvik Markus,
Jantson Tanel,
Lakso Merja,
Callaway J. C.,
Wong Garry
Publication year - 2011
Publication title -
journal of biochemical and molecular toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.526
H-Index - 58
eISSN - 1099-0461
pISSN - 1095-6670
DOI - 10.1002/jbt.20386
Subject(s) - caenorhabditis elegans , polyunsaturated fatty acid , mutant , wild type , biology , metabolism , endocrinology , medicine , gene , biochemistry , food science , fatty acid
Trans‐fatty acids (TFAs) enter the diet through industrial processes and can cause adverse human health effects. The present study was aimed to examine the effects of dietary cis‐ and trans‐fatty acids on the model organism Caenorhabditis elegans . Cis ‐ or trans ‐18:1n9 triglycerides (25 μM) caused no apparent changes in the numbers of viable progeny of wild‐type N2 animals. However, in fat ‐3 mutants lacking delta ‐6‐desaturase, the trans‐isomer caused modest decreases in lifespan and progeny after three generations. Long‐chain polyunsaturated fatty acids (PUFA) profiles were significantly altered in fat ‐3 mutants compared to wild type but were not altered after exposure to dietary cis ‐ or trans ‐18:1n9. Genome‐wide expression analysis of fat ‐3 mutants revealed hundreds of changes. Several genes involved in fat metabolism ( acs ‐2, fat ‐7, mdt ‐15) were significantly increased by cis ‐ or trans ‐18:1n9 without discrimination between isomers. These results provide support for the hypothesis that dietary trans fats are detrimental to development and aging. © 2011 Wiley Periodicals, Inc. J Biochem Mol Toxicol 25:269–279, 2011; View this article online at wileyonlinelibrary.com . DOI 10.1002/jbt.20386