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An assessment of transcriptional changes in porcine skin exposed to bromine vapor
Author(s) -
Rogers James V.,
Price Jennifer A.,
Wendling Morgan Q. S.,
Perry Mark R.,
Reid Frances M.,
Kiser Robyn C.,
Graham John S.
Publication year - 2011
Publication title -
journal of biochemical and molecular toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.526
H-Index - 58
eISSN - 1099-0461
pISSN - 1095-6670
DOI - 10.1002/jbt.20383
Subject(s) - bromine , chemistry , microbiology and biotechnology , biology , organic chemistry
Bromine is an industrial chemical that can cause severe cutaneous burns. This study was a preliminary investigation into the effect of cutaneous exposure to bromine vapor using a weanling swine burn model and microarray analysis. Ventral abdominal sites were exposed to a mean calculated bromine vapor concentration of 0.69 g L −1 for 10 or 20 min. At 48 h postexposure, total RNA from skin samples was isolated, processed, and hybridized to Affymetrix GeneChip Porcine Genome Arrays. Expression analysis revealed that bromine vapor exposure for 10 or 20 min promoted similar transcriptional changes in the number of significantly modulated probe sets. A minimum of 83% of the probe sets was similar for both exposure times. Ingenuity pathways analysis revealed eight common biological functions among the top 10 functions of each experimental group, in which 30 genes were commonly shared among 19 significantly altered signaling pathways. Transcripts encoding heme oxygenase 1, interleukin‐1β, interleukin 2 receptor gamma chain, and plasminogen activator inhibitor‐1 were identified as common potential therapeutic targets for Phase II/III clinical trial or FDA‐approved drugs. The present study is an initial assessment of the transcriptional responses to cutaneous bromine vapor exposure identifying molecular networks and genes that could serve as targets for developing therapeutics for bromine‐induced skin injury. © 2011 Wiley Periodicals, Inc. J Biochem Mol Toxicol 25:252–262, 2011; View this article online at wileyonlinelibrary.com . DOI 10.1002/jbt.20383