Premium
Effect of tin and lead chlorotriphenyl analogues on selected living cells
Author(s) -
BoniewskaBernacka Ewa,
Man Dariusz,
Słota Rudolf,
Broda Małgorzata A.
Publication year - 2010
Publication title -
journal of biochemical and molecular toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.526
H-Index - 58
eISSN - 1099-0461
pISSN - 1095-6670
DOI - 10.1002/jbt.20380
Subject(s) - tin , membrane , saccharomyces cerevisiae , chemistry , liposome , lipid bilayer , membrane fluidity , biophysics , electron paramagnetic resonance , yolk , cell membrane , cell , lecithin , biochemistry , yeast , biology , organic chemistry , food science , nuclear magnetic resonance , physics
Three kinds of living cells, human embryonic kidney cells , Saccharomyces cerevisiae , and Escherichia coli , were tested for their sensitivity to chlorotriphenyltin and chlorotriphenyllead. The tin compound proved definitely more toxic than the lead derivative, particularly in the case of the human embryonic kidney cells devoid of any protective cell wall. Electron paramagnetic resonance (EPR) comparative studies carried out by using a natural model liposome system (egg yolk lecithin) confirmed considerable changes within the lipid bilayer upon doping by the aforementioned additives, which may be crucial to the mechanism of the observed cell cleavage. The individual dopants revealed diverse impact upon the membrane's condition, chlorotriphenyltin distinctly fluidized the lipid system, whereas chlorotriphenyllead stiffened the medium within the membrane. A theoretical approach concerning such different behaviors of studied tin and lead analogues because of their high toxicity in living cells has been presented. © 2010 Wiley Periodicals, Inc. J Biochem Mol Toxicol 25:231–237, 2011; View this article online at wileyonlinelibrary.com . DOI 10.1002/jbt.20380