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Proteomic analysis of urine in rats chronically exposed to fluoride
Author(s) -
Kobayashi Claudia Ayumi Nakai,
Leite Aline de Lima,
Silva Thelma Lopes da,
Santos Lucilene Delazari dos,
Nogueira Fábio César Sousa,
Santos Keity Souza,
Oliveira Rodrigo Cardoso de,
Palma Mario Sérgio,
Domont Gilberto Barbosa,
Buzalaf Marília Afonso Rabelo
Publication year - 2011
Publication title -
journal of biochemical and molecular toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.526
H-Index - 58
eISSN - 1099-0461
pISSN - 1095-6670
DOI - 10.1002/jbt.20353
Subject(s) - urine , chemistry , fluoride , chromatography , pharmacology , medicine , biochemistry , inorganic chemistry
Urine is an ideal source of materials to search for potential disease‐related biomarkers as it is produced by the affected tissues and can be easily obtained by noninvasive methods. 2‐DE‐based proteomic approach was used to better understand the molecular mechanisms of injury induced by fluoride (F − ) and define potential biomarkers of dental fluorosis. Three groups of weanling male Wistar rats were treated with drinking water containing 0 (control), 5, or 50 ppm F − for 60 days ( n = 15/group). During the experimental period, the animals were kept individually in metabolic cages, to analyze the water and food consumption, as well as fecal and urinary F − excretion. Urinary proteome profiles were examined using 2‐DE and Colloidal Coomassie Brilliant Blue staining. A dose‐response regarding F − intake and excretion was detected. Quantitative intensity analysis revealed 8, 11, and 8 significantly altered proteins between control vs. 5 ppm F − , control vs. 50 ppm F − and 5 ppm F − vs. 50 ppm F − groups, respectively. Two proteins regulated by androgens (androgen‐regulated 20‐KDa protein and α‐2μ‐globulin) and one related to detoxification (aflatoxin‐B1‐aldehyde‐reductase) were identified by MALDI‐TOF‐TOF MS/MS. Thus, proteomic analysis can help to better understand the mechanisms underlying F − toxicity, even in low doses. © 2010 Wiley Periodicals, Inc. J Biochem Mol Toxicol 25:8–14 2011; View this article online at wileyonlinelibrary.com . DOI 10.1002/jbt.20353