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Protective role of withaferin‐A on 7,12‐dimethylbenz(a)anthracene‐induced genotoxicity in bone marrow of Syrian golden hamsters
Author(s) -
Panjamurthy K.,
Manoharan S.,
Me Venugopal P.,
Nirmal Madhavan R.,
Senthil N.
Publication year - 2008
Publication title -
journal of biochemical and molecular toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.526
H-Index - 58
eISSN - 1099-0461
pISSN - 1095-6670
DOI - 10.1002/jbt.20235
Subject(s) - dmba , genotoxicity , withaferin a , bone marrow , chemistry , 7,12 dimethylbenz[a]anthracene , toxicology , pharmacology , biology , toxicity , withania somnifera , biochemistry , immunology , medicine , pathology , organic chemistry , carcinogenesis , alternative medicine , gene
The present study has investigated the antigenotoxic effect of withaferin‐A, a steroidal lactone obtained from the roots and leaves of Withania somnifera , in 7,12‐dimethylbenz(a)anthracene (DMBA)‐induced genotoxicity. Measurement of the frequency of micronucleated polychromatic erythrocytes (MnPCEs) and chromosomal aberrations is used as cytogenetic endpoints. A single intraperitoneal injection of DMBA (30 mg/kg b.w.) to golden Syrian hamsters resulted in marked elevation in the frequency of MnPCEs and aberrations in the chromosomal structure. Hamsters pretreated with withaferin‐A intraperitonealy 2 h before the injection of DMBA, significantly reduced the frequency of MnPCEs and chromosomal aberrations such as chromosomal break, gap, minute, and fragment. Our results thus demonstrated the antigenotoxic effect of withaferin‐A in DMBA‐induced genotoxicity in the bone marrow of golden Syrian hamsters. © 2008 Wiley Periodicals, Inc. J Biochem Mol Toxicol 22:251–258, 2008; Published online in Wiley InterScience ( www.interscience.wiley.com ). DOI 10.1002/jbt.20235