Liver prenylated methylated protein methyl esterase is the same enzyme as sus scrofa carboxylesterase

The C‐terminal COOH of prenylated proteins is methylated to COOCH 3 . The COOCH 3 ester forms are hydrolyzed by prenylated methylated protein methyl esterase (PMPMEase) to the original acid forms. This is the only reversible step of the prenylation pathway. PMPMEase has not been purified and identified and is therefore understudied. Using a prenylated‐L‐cysteine methyl ester as substrate, PMPMEase was purified to apparent homogeneity from porcine liver supernatant. SDS‐PAGE analysis revealed an apparent mass of 57 kDa. Proteomics analyses identified 17 peptides (242 amino acids). A Mascot database search revealed these as portions of the Sus scrofa carboxylesterase, a 62‐kDa serine hydrolase with the C‐terminal HAEL endoplasmic reticulum‐retention signal. It is at least 71% identical to such mammalian carboxylesterases as human carboxylesterase 1 with affinities toward hydrophobic substrates and known to activate prodrugs, metabolize active drugs, as well as detoxify various substances such as cocaine and food‐derived esters. The purified enzyme hydrolyzed benzoyl‐Gly‐farnesyl‐L‐cysteine methyl ester and hydrocinamoyl farnesyl‐L‐cysteine methyl ester with Michaelis–Menten constant ( K m ) values of 33 ± 4 and 25 ± 4 μM and V max values of 4.51 ± 0.28 and 6.80 ± 0.51 nmol/min/mg of protein, respectively. It was inhibited by organophosphates, chloromethyl ketones, ebelactone A and B, and phenylmethylsulfonyl fluoride. © 2008 Wiley Periodicals, Inc. J Biochem Mol Toxicol 22:51–62, 2008; Published online in Wiley InterScience ( www.interscience.wiley.com ). DOI 10.1002/jbt.20214