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Preventive effect of naringin on cardiac mitochondrial enzymes during isoproterenol‐induced myocardial infarction in rats: A transmission electron microscopic study
Author(s) -
Rajadurai M.,
Stanely Mainzen Prince P.
Publication year - 2007
Publication title -
journal of biochemical and molecular toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.526
H-Index - 58
eISSN - 1099-0461
pISSN - 1095-6670
DOI - 10.1002/jbt.20203
Subject(s) - naringin , succinate dehydrogenase , myocardial infarction , mitochondrion , cytochrome c oxidase , enzyme , dehydrogenase , chemistry , malate dehydrogenase , medicine , citric acid cycle , endocrinology , biochemistry , chromatography
Abstract Dietary flavonoids intake has been reported inversely related to the incidence of cardiovascular diseases (CVD). The present study is undertaken to evaluate the preventive role of naringin on mitochondrial enzymes in isoproterenol (ISO)‐induced myocardial infarction in male albino Wistar rats. Rats subcutaneously injected with ISO (85 mg/kg) at an interval of 24 h for 2 days, resulting in significant ( p < 0.05) increase in the levels of mitochondrial lipid peroxides. ISO‐induction also showed significant ( p < 0.05) decrease in the activities of mitochondrial tricarboxylic acid cycle enzymes (isocitrate dehydrogenase, succinate dehydrogenase, malate dehydrogenase, and α‐ketoglutarate dehydrogenase) and respiratory chain enzymes (NADH dehydrogenase and cytochrome c oxidase). Oral pretreatment with naringin (10, 20, and 40 mg/kg) to ISO‐induced rats daily for a period of 56 days significantly ( p < 0.05) minimized the alterations in all the biochemical parameters and restored the normal mitochondrial function. Transmission electron microscopic (TEM) observations also correlated with these biochemical findings. Thus, our findings demonstrate that naringin prevents the mitochondrial dysfunction during ISO‐induced myocardial infarction in rats. © 2007 Wiley Periodicals, Inc. J Biochem Mol Toxicol 21:354–361, 2007; Published online in Wiley InterScience ( www.interscience.wiley.com ). DOI 10.1002/jbt.20203