Preventive effect of S ‐allyl cysteine sulfoxide (alliin) on lysosomal hydrolases and membrane‐bound atpases in isoproterenol‐induced myocardial infarction in wistar rats

In this study, S ‐allyl cysteine sulfoxide (SACS) was used to evaluate its preventive effect in isoproterenol (ISO)‐induced myocardial ischemia in male Wistar rats. Rats were pretreated with SACS (40 and 80 mg kg −1 ) orally for 5 weeks. After the treatment period, ISO (150 mg kg −1 ) was administered subcutaneously to rats at an interval of 24 h for 2 days. The activities of β‐D‐ N ‐acetyl‐glucosaminidase, β‐galactosidase, β‐glucosidase, and acid phosphatase increased in serum and heart in ISO‐induced rats. In addition, these rats showed a significant ( p < 0.05) increase in the activities of β‐glucuronidase and cathepsin‐D in serum and heart and a significant ( p < 0.05) decrease in their activities in lysosomal fraction of the heart. The activity of Na + K + ‐ATPase declined, while those of Ca 2+ ‐ and Mg 2+ ‐ATPases significantly ( p < 0.05) elevated in the heart of ISO‐induced rats. Pretreatment with SACS (40 and 80 mg kg −1 ) showed a significant ( p < 0.05) effect in all the biochemical parameters studied. The effect at a dose of 80 mg kg −1 body weight was more effective than that at 40 mg kg −1 body weight and brought back all the biochemical parameters to near normal levels. Hereby, our study shows the membrane‐stabilizing as well as antioxidant effects of SACS in ISO‐induced rats. © 2007 Wiley Periodicals, Inc. J Biochem Mol Toxicol 21:118–124, 2007; Published online in Wiley InterScience ( www.interscience.wiley.com ). DOI 10.1002/jbt.20166