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Vitamin E supplementation alters HDL‐cholesterol concentration and paraoxonase activity in rabbits fed high‐cholesterol diet: Comparison with probucol
Author(s) -
Jeon SeonMin,
Park Yong Bok,
Kwon OhShin,
Huh TaeLin,
Lee WonHa,
Do KyungMin,
Park Taesun,
Choi MyungSook
Publication year - 2005
Publication title -
journal of biochemical and molecular toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.526
H-Index - 58
eISSN - 1099-0461
pISSN - 1095-6670
DOI - 10.1002/jbt.20098
Subject(s) - probucol , medicine , endocrinology , paraoxonase , chemistry , cholesterol , vitamin , vitamin e , high density lipoprotein , apolipoprotein b , lipoprotein , antioxidant , oxidative stress , biochemistry , biology
Vitamin E and probucol are well‐known antioxidants that prevent cells from the oxidative stress, which is a risk factor of atherosclerosis. Male rabbits were fed either 0.03% vitamin E or 0.05% probucol in a 0.5% high‐cholesterol (HC) diet for 8 weeks. Vitamin E and probucol significantly suppressed an increase in plasma total‐cholesterol (total‐C) and low‐density lipoprotein cholesterol compared to HC‐control group. However, plasma high‐density lipoprotein‐cholesterol (HDL‐C) and HDL‐C/total‐C ratio levels and plasma paraoxonase activity were only significantly higher in vitamin E group after 8 weeks. Hepatic ACAT activity was significantly lower in both vitamin E and probucol groups than in HC‐control group, while HMG‐CoA reductase activity was the highest only in the probucol group. Total fecal sterol content was significantly higher in probucol and vitamin E groups than in the two control groups. Some atherogenic signs were discovered in the aortic fatty streak of HC‐control group, yet not in other groups. Hepatic mRNA expressions of apo B‐100 and apo C‐III were significantly lower in probucol group than in other groups. Vitamin E supplementation was found to alter the plasma HDL‐C‐related factors; meanwhile, probucol supplementation was very effective in enhancing cholesterol metabolism, except for a negative effect that reduced plasma HDL‐C concentration. © 2005 Wiley Periodicals, Inc. J Biochem Mol Toxicol 19:336–346, 2005; Published online in Wiley InterScience ( www.interscience.wiley.com ). DOI 10.1002/jbt.20098