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Carbon monoxide and oxidative stress in Desulfovibrio desulfuricans B‐1388
Author(s) -
Davydova M.,
Sabirova R.,
Vylegzhani.,
Tarasova N.
Publication year - 2004
Publication title -
journal of biochemical and molecular toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.526
H-Index - 58
eISSN - 1099-0461
pISSN - 1095-6670
DOI - 10.1002/jbt.20011
Subject(s) - oxidative stress , carbon monoxide , chemistry , biochemistry , catalysis
It has been shown that carbon monoxide (CO) in low concentration may be an active biochemical and physiological regulator of cell function. The bases of CO toxicity and cell protection are not clearly understood. To provide insights into these mechanisms, we measured superoxide production by D. desulfuricans B‐1388 incubated anaerobically in Postgate medium with or without 5% CO. D. desulfuricans B‐1388 growing with CO in the gas phase produced more superoxide radicals then control cells growing in Ar. When the cells were pregrown with CO, NADH oxidase and peroxidase activities were increased. The increase in peroxidase activities of cells growing under CO (particularly NADH peroxidase) suggested that H 2 O 2 was accumulated in cells. Superoxide dismutase (SOD) activity of cells decreased in exponential growth phase and increased in stationary phase. This may be due to CO concentration fall during CO oxidation by CO dehydrogenase. Altogether, our data suggest that superoxide production is a possible mechanism of CO toxicity. © 2004 Wiley Periodicals, Inc. J Biochem Mol Toxicol 18:87–91, 2004; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/jbt.20011