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Characterization of triacsin C inhibition of short‐, medium‐, and long‐chain fatty acid:CoA ligases of human liver
Author(s) -
Vessey Donald A.,
Kelley Michael,
Warren Robert S.
Publication year - 2004
Publication title -
journal of biochemical and molecular toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.526
H-Index - 58
eISSN - 1099-0461
pISSN - 1095-6670
DOI - 10.1002/jbt.20009
Subject(s) - dna ligase , biochemistry , fatty acid , enzyme , biology , substrate (aquarium) , long chain fatty acid , microsome , fatty acid metabolism , chemistry , microbiology and biotechnology , ecology
Short‐, medium‐, and long‐chain fatty acid:CoA ligases from human liver were tested for their sensitivity to inhibition by triacsin C. The short‐chain fatty acid:CoA ligase was inhibited less than 10% by concentrations of triacsin C as high as 80 μM. The two mitochondrial xenobiotic/medium‐chain fatty acid:CoA ligases (XM‐ligases), HXM‐A and HXM‐B, were partially inhibited by triacsin C, and the inhibitions were characterized by low affinity for triacsin C ( K I values > 100 μM). These inhibitions were found to be the result of triacsin C competing with medium‐chain fatty acid for binding at the active site. The microsomal and mitochondrial forms of long‐chain fatty acid:CoA ligase (also termed long‐chain fatty acyl‐CoA synthetase, or long‐chain acyl‐CoA synthetase LACS) were potently inhibited by triacsin C, and the inhibition had identical characteristics for both LACS forms. Dixon plots of this inhibition were biphasic. There is a high‐affinity site with a K I of 0.1 μM that accounts for a maximum of 70% of the inhibition. There is also a low affinity site with a K I of 6 μM that accounts for a maximum of 30% inhibition. Kinetic analysis revealed that the high‐affinity inhibition of the mitochondrial and microsomal LACS forms is the result of triacsin C binding at the palmitate substrate site. The high‐affinity triacsin C inhibition of both the mitochondrial and microsomal LACS forms was found to require a high concentration of free Mg 2+ , with the EC 50 for inhibition being 3 mM free Mg 2+ . The low affinity triacsin C inhibition was also enhanced by Mg 2+ . The data suggests that Mg 2+ promotes triacsin C inhibition of LACS by enhancing binding at the palmitate binding site. In contrast, the partial inhibition of the XM‐ligases by triacsin C, which showed only a low‐affinity component, did not require Mg 2+ . © 2004 Wiley Periodicals, Inc. J Biochem Mol Toxicol 18:100–106, 2004; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/jbt.20009