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Organophosphorous and organochlorine pesticides affect human placental phosphoinositides metabolism and PI‐4 kinase activity
Author(s) -
Souza María S.,
de Potas Gladis Magnarelli,
de D'Angelo Ana M. Pechén
Publication year - 2004
Publication title -
journal of biochemical and molecular toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.526
H-Index - 58
eISSN - 1099-0461
pISSN - 1095-6670
DOI - 10.1002/jbt.20003
Subject(s) - phosphatidylinositol , kinase , metabolism , biochemistry , pesticide , inositol , enzyme , chemistry , biology , receptor , agronomy
The objective of this work was to describe the effect of organophosphorous and organochlorine pesticides on phosphoinositides metabolism in human placenta. Pesticides concentration (10 μM) was used for in vitro incubations of cell‐free homogenates labelled with 32 P orthophosphate. Heptachlor (HC) and dichloro‐diphenyl‐trichloroethane ( o‐p ′ DDT) increased phosphatidyl‐inositol, phosphatidylinositolphosphate, and phosphatidyl‐inositolbiphosphate phosphorylation while azinphosmethyl (AM) increased phosphatidylinositolbiphosphate labeling. Decreased 32 P incorporation in phosphatidylinositol was found with phosmet (PM), AM, and chlorpyriphos (CHL). The effects of these xenobiotics on PI4‐kinase activity using different subcellular fractions were also examined. Both type of pesticides affected the postmembrane supernatant enzyme activity. A biphasic effect on membrane and nuclear PI4‐kinase activity was seen with HC. The strongest effect found was seen with o‐p ′ DDT in nuclear kinase activity while substantial changes were also observed in membrane. These data demonstrate the sensitivity of human placental PI4‐kinase to pesticides currently found in human tissues and suggest deleterious consequences in different processes regulated by 4‐phosphoinositides. © 2004 Wiley Periodicals, Inc. J Biochem Mol Toxicol 18:30–36, 2004; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/jbt.20003