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Protein expression pattern of P–glycoprotein along the gastrointestinal tract of the yucatan micropig
Author(s) -
Tang Huadong,
Pak Yvonne,
Mayersohn Michael
Publication year - 2004
Publication title -
journal of biochemical and molecular toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.526
H-Index - 58
eISSN - 1099-0461
pISSN - 1095-6670
DOI - 10.1002/jbt.20001
Subject(s) - western blot , ileum , duodenum , small intestine , gastrointestinal tract , glycoprotein , p glycoprotein , microbiology and biotechnology , biology , chemistry , medicine , biochemistry , multiple drug resistance , gene , antibiotics
The purpose of this study is to characterize the distribution pattern of P–gp protein levels along the entire GI tract in the Yucatan micropig, which is being developed as a model for human drug bioavailability. Small and large intestines were freshly obtained and divided into about 37 segments and 10 segments, respectively (ca., 1 foot/segment). Epithelial cells from the small intestine were obtained by an elution method; whereas, a scraping method was applied to the large intestine. Total cellular protein was isolated from the epithelial cells. Western blot analysis using P–gp antibody showed that the amount of P–gp protein increased distally from the duodenum to the ileum over approximately a 10–fold range. P–gp protein in the large intestine was present at a higher level in the central portion, but the absolute amount was much less than what was found in the small intestine. © 2004 Wiley Periodicals, Inc. J Biochem Mol Toxicol 18:18–22, 2004; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/jbt.20001