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Direct effect of phosphorus on PTH secretion from whole rat parathyroid glands in vitro
Author(s) -
Almaden Yolanda,
Canalejo Antonio,
Hernandez Alexis,
Ballesteros Evaristo,
GarciaNavarro Socorro,
Torres Armando,
Rodriguez Mariano,
Rodriguez Mariano
Publication year - 1996
Publication title -
journal of bone and mineral research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.882
H-Index - 241
eISSN - 1523-4681
pISSN - 0884-0431
DOI - 10.1002/jbmr.5650110714
Subject(s) - parathyroid hormone , endocrinology , medicine , calcium , calcitriol , parathyroid chief cell , phosphorus , chemistry , hyperparathyroidism , secretion , calcium metabolism , biology , organic chemistry
Phosphorus retention is an important factor in the development of hyperparathyroidism secondary to renal failure. In vivo manipulation of phosphorus is associated with changes in serum calcium and calcitriol levels which in turn can modify parathyroid hormone synthesis and secretion. The present in vitro study evaluates whether high extracellular phosphorus has a direct effect on parathyroid hormone secretion. Fresh rat parathyroid glands were incubated in a media with phosphorus concentrations of 1, 2, 3, and 4 mM and subsequently exposed to calcium levels ranging from 0.4 to 1.35 mM. In 1.25 mM calcium, the parathyroid hormone secretion rate was similar in 1 and 2 mM phosphorus; however, a phosphorus concentration of 3 and 4 mM produced a 3‐ and 4‐fold increase in the parathyroid hormone secretion, respectively, as compared with 1 mM phosphorus. While in 1 or 2 mM phosphorus an increase in calcium from 0.6 to 1.35 mM reduced parathyroid hormone secretion to 37%, in 4 mM phosphorus the same increase in calcium only inhibited parathyroid hormone secretion to 75%. Furthermore, the addition of arachidonic acid 20 μM, a substrate for inhibitory intracellular signal pathway, to the 4 mM phosphorus‐1.35 mM calcium incubation media reduced the parathyroid hormone secretion to 34.5% ( p < 0.05). In conclusion, our results indicate that in vitro, high phosphorus directly increases parathyroid hormone secretion. (J Bone Miner Res 1996;11:970‐976)