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Identification and cloning of human P 2U purinoceptor present in osteoclastoma, bone, and osteoblasts
Author(s) -
Bowler W.B.,
Birch M.A.,
Gallagher J.A.,
Bilbe G.
Publication year - 1995
Publication title -
journal of bone and mineral research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.882
H-Index - 241
eISSN - 1523-4681
pISSN - 0884-0431
DOI - 10.1002/jbmr.5650100720
Subject(s) - purinergic receptor , bone remodeling , microbiology and biotechnology , flow cytometry , biology , bone cell , population , extracellular , endocrinology , medicine , environmental health
Extracellular ATP acting through purinoceptors may be an important factor in the modulation of bone turnover. In this study we cloned and sequenced the P 2U purinoceptor from osteoclastoma, confirming the recently published human sequence. Furthermore, by the reverse transcriptase‐linked polymerase chain reaction (RT‐PCR) and Southern blotting we demonstrated expression of P 2U receptor mRNA in bone, primary cultures of human bone‐derived cells, and two osteosarcoma cell lines, Saos2 and Te85. P 2U receptor transcripts were identified in alkaline phosphatase‐positive human bone‐derived cells isolated by flow cytometry providing strong evidence for the expression of the P 2U purinoceptor in mature osteoblasts. P 2U receptor transcripts were also detected in a purified giant cell population isolated from osteoclastoma, indicating that this receptor is also expressed by osteoclasts. These data suggest that purinergic agonists may play a role in the regulation of bone metabolism.