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Surface‐associated proteins from Staphylococcus aureus demonstrate potent bone resorbing activity
Author(s) -
Nair Sean,
Song Yu,
Meghji Sajeda,
Reddi Kris,
Harris Malcolm,
Ross Alistair,
Poole Stephen,
Wilson Michael,
Henderson Brian
Publication year - 1995
Publication title -
journal of bone and mineral research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.882
H-Index - 241
eISSN - 1523-4681
pISSN - 0884-0431
DOI - 10.1002/jbmr.5650100509
Subject(s) - bone resorption , staphylococcus aureus , resorption , lipoteichoic acid , chemistry , microbiology and biotechnology , medicine , biology , bacteria , genetics
Staphylococcus aureus infections are associated with rapid bone destruction in conditions such as osteomyelitis, bacterial arthritis, and infected orthopedic implant failure. How this bacterium induces bone destruction has not been defined. In studies of the role of oral Gram‐negative bacteria in periodontal pathology, we have established that cell surface‐associated proteins (SAPs) are potent stimulators of bone resorption. The surface‐associated components from S. aureus have now been isolated and demonstrated to be extremely potent stimulators of bone resorption in the murine calvarial bone resorption assay. Bone resorption appears to be due to proteins, is not the result of contamination with lipoteichoic acid or muramvl dipeptide, and is potently inhibited by indomethacin and can be completely blocked by high concentrations of interleukin‐1 receptor antagonist or TN3–19.12, a neutralizing monoclonal antibody to murine TNF. The SAP fraction can stimulate fibroblasts or monocytes to release osteolytic cytokines, but only at high concentrations. Fractionation of the SAPs by high performance liquid chromatography demonstrated that a number of fractions were osteolytically active. The most active contained a heterodimeric protein of molecular weight 32–36 kD. The presence of this osteolytically active surface‐associated fraction may account for the bone resorption associated with local infection with S. aureus.