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Three‐dimensional type I collagen gel system for the study of osteoblastic metastases produced by metastatic prostate cancer
Author(s) -
Koutsilieris M.,
Sourla A.,
Pelletier G.,
Doillon C.J.
Publication year - 1994
Publication title -
journal of bone and mineral research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.882
H-Index - 241
eISSN - 1523-4681
pISSN - 0884-0431
DOI - 10.1002/jbmr.5650091120
Subject(s) - cancer cell , cell culture , microbiology and biotechnology , immunocytochemistry , polyclonal antibodies , osteoblast , chemistry , plasminogen activator , in vitro , pathology , cancer research , biology , antibody , cancer , immunology , endocrinology , medicine , biochemistry , genetics
We developed a three‐dimensional type I collagen gel cell culture system that allows coculturing of human MG‐63 osteoblast‐like cells and various human cancer cells. Inoculation of human PC‐3 metastatic prostate cancer cells into this type I collagen gel containing human MG‐63 osteoblast‐like cells produced an osteoblastic‐like reaction that presented as an increased number of MG‐63 cells and increased density of type I collagen around MG‐63 cells adjacent to inoculated PC‐3 cells by microscope analysis. Under identical experimental conditions, inoculation of cell‐free medium, human KLE endometrial adenocarcinoma cells, and Calu‐1 lung cancer cells did not produce this blastic‐like reaction. In situ hybridization documented the uniform expression of insulin‐like growth factor I (IGF‐I) and of urokinase‐type plasminogen activator (uPA) mRNA in MG‐63 and PC‐3 cells separately cultured in this substrata. The uniform expression of uPA was also documented by immunocytochemistry using a monoclonal and a polyclonal antihuman uPA antibody. The relative expression of uPA was higher in PC‐3 cells than in MG‐63, KLE, and Calu‐1 cancer cells. We conclude that this novel cell culture system may become a useful model to study the pathophysiology of the osteoblastic reaction in vitro.