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Monoclonal antibodies with selective reactivity against osteoblasts and osteocytes in human bone
Author(s) -
Walsh Susan,
Dodds Robert A.,
James Ian E.,
Bradbeer Jeremy N.,
Gowen Maxine
Publication year - 1994
Publication title -
journal of bone and mineral research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.882
H-Index - 241
eISSN - 1523-4681
pISSN - 0884-0431
DOI - 10.1002/jbmr.5650091104
Subject(s) - osteoblast , monoclonal antibody , immunogen , immunohistochemistry , antibody , chemistry , microbiology and biotechnology , bone cell , biology , in vitro , pathology , immunology , biochemistry , medicine
Monoclonal antibodies (MAb) may provide valuable tools for studying osteoblast differentiation. We therefore raised a panel of MAb reactive with cells of this phenotype using 1,25(OH) 2 D 3 ‐treated human trabecular osteoblast‐like cells (HOBS) as the immunogen. Immunohistochemical studies on various tissues, including undecalcified cryostat sections of fetal and adult human bone, identified 11 bone cell‐reactive MAb. Of these, 2 demonstrated particularly selective reactivities against osteocytes (OB/M) and osteoblasts (OB/L). These reactivities were also seen in developing bone from rat, rabbit, and marmoset. OB/L and OB/M demonstrated limited reactivity against a small number of human tissues from the extensive panel of substrates tested. Both MAb exhibited reactivity against discrete populations of cells in the large and small intestine. In addition, OB/L reacted with cells in the basal epidermis of skin and OB/M with cells in blood vessel walls. Both antibodies demonstrated reactivity against a variety of cultured osteoblast‐like cell lines and other cultured cell types. These MAb may therefore provide a valuable means of studying osteoblast ontogeny.