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Localization of the mRNA for bone matrix proteins during fracture healing as determined by in situ hybridization
Author(s) -
Hirakawa Kimiaki,
Hirota Sehchi,
Ikeda Tohru,
Yamaguchi Akira,
Takemura Teiji,
Nagoshi Junsuke,
Yoshiki Shusaku,
Suda Tatsuo,
Kitamura Yukihiko,
Nomura Shintaro
Publication year - 1994
Publication title -
journal of bone and mineral research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.882
H-Index - 241
eISSN - 1523-4681
pISSN - 0884-0431
DOI - 10.1002/jbmr.5650091007
Subject(s) - osteopontin , osteonectin , in situ hybridization , osteocalcin , periosteum , bone healing , messenger rna , osteoblast , chondrogenesis , osteocyte , chemistry , matrix (chemical analysis) , bone cell , microbiology and biotechnology , anatomy , biology , endocrinology , cartilage , alkaline phosphatase , in vitro , gene , biochemistry , chromatography , enzyme
The expression of the mRNAs for osteonectin (ON), osteopontin (OPN), osteocalcin (OC), and matrix Gla protein (MGP) was studied by in situ hybridization during the healing process of an experimental fracture in adult rat femora. At day 1 postoperatively, ON mRNA was detected in the proliferating periosteum. At day 3, ON, OPN, and OC mRNAs were detected in woven bone. From day 5, MGP and ON mRNAs were detected in the immature chondrocytes. From day 7, ON, OPN, and OC mRNAs were detected in the osteoblastic cells in newly formed endosteal trabecular bone. OPN mRNA was also detected in some of the osteocytes in trabecular bone. From day 14, OPN and MGP mRNAs were detected in newly formed periosteal hypertrophic chondrocytes, and the ON, OPN, and OC mRNAs were detected in osteoblastic cells in newly formed periosteal trabecular bone. Although the cell types that expressed each mRNA in fractured bones were similar to those in embryonic bones, the time course of these mRNA expression in fractured bones was different from that in embryonic bones. We considered that this system is useful to investigate the phenotypic change in osteogenic and chondrogenic lineage cells that appears during fracture healing at the molecular level.