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Carboxyl‐terminal parathyroid hormone peptide (53–84) elevates alkaline phosphatase and osteocalcin mRNA levels in SaOS‐2 cells
Author(s) -
Sutherland M. Kung,
Rao L.G.,
Wylie J.N.,
Gupta A.,
Ly H.,
Sodek J.,
Murray T.M.
Publication year - 1994
Publication title -
journal of bone and mineral research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.882
H-Index - 241
eISSN - 1523-4681
pISSN - 0884-0431
DOI - 10.1002/jbmr.5650090403
Subject(s) - alkaline phosphatase , osteocalcin , endocrinology , medicine , parathyroid hormone , osteoblast , type i collagen , chemistry , messenger rna , biology , enzyme , in vitro , biochemistry , calcium , gene
Previous findings in our laboratory have shown that hPTH‐(53–84) stimulates alkaline phosphatase activity in dexamethasone‐treated ROS 17/2.8 cells. In the present study, we examined the effects of hPTH‐(53–84) and hPTH‐(1–34) on the expressions of alkaline phosphatase, osteocalcin, and collagen type I mRNA levels in the human osteosarcoma cell line SaOS‐2. The effect of hPTH‐(53–84) on alkaline phosphatase and osteocalcin message levels was dose dependent (ANOVA, p < 0.005 and p < 0.001, respectively), with significant stimulation observed at 10 nM. Treatment with 10 nM hPTH‐(53–84) for 24 h resulted in significant 2‐ and 1.4‐fold increases in mRNA levels for alkaline phosphatase and osteocalcin, respectively ( p < 0.05), but had no effect on collagen type I expression. The increased alkaline phosphatase mRNA levels was associated with a 1.5‐fold increase in enzyme activity ( p < 0.05). In contrast, under similar incubation conditions, hPTH‐(1–34) had no significant effects on alkaline phosphatase or osteocalcin mRNA levels. On the other hand, hPTH‐(1–34) had dose‐dependent stimulatory effects on collagen type I mRNA levels (ANOVA, p < 0.001), 10 nM hPTH‐(1–34) stimulating collagen type I expression 1.6‐fold ( p < 0.05). The results indicate that carboxyl‐terminal hPTH‐(53–84) has direct and unique biologic effects in human osteoblast‐like cells in culture.