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Macrophage colony‐stimulating factor restores bone resorption in op/op bone in vitro in conjunction with parathyroid hormone or 1,25‐dihydroxyvitamin D 3
Author(s) -
Morohashi Tomio,
Corboz Véronique Antonioli,
Fleisch Herbert,
Cecchini Marco G.,
Felix Rolf
Publication year - 1994
Publication title -
journal of bone and mineral research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.882
H-Index - 241
eISSN - 1523-4681
pISSN - 0884-0431
DOI - 10.1002/jbmr.5650090316
Subject(s) - bone resorption , osteoclast , medicine , endocrinology , parathyroid hormone , macrophage colony stimulating factor , resorption , chemistry , haematopoiesis , bone remodeling , macrophage , in vitro , biology , calcium , microbiology and biotechnology , stem cell , receptor , biochemistry
The in vivo administration of macrophage colony‐stimulating factor (M‐CSF) restores osteoclastogenesis and bone resorption in the op/op murine osteopetrosis. In vitro, exogenous M‐CSF has been shown to be necessary for the generation of osteoclast‐like cells in cocultures of hematopoietic and mesenchymal cells obtained from this mutant. In this study we investigated the capacity of M‐CSF and other cytokines and hormones, alone or in combination, to induce bone resorption in explants of op/op metatarsals and metacarpals prelabeled with 45 Ca. The effect on bone resorption was verified by counting the number of osteoclasts generated in the mineralized matrix. No osteoclast formation and no bone resorption were observed in the absence of M‐CSF. M‐CSF alone had only a slight effect at the high concentration of 10 4 units/ml. Addition of PTH or 1,25‐(OH) 2 D 3 together with M‐CSF induced both osteoclastogenesis and bone resorption. The release of 45 Ca was linear with time up to 15 days. PTH or 1,25‐(OH) 2 D 3 could not be substituted by TNF‐α or IL‐1, whereas IL‐6 had a weak effect. M‐CSF could not be replaced by GM‐CSF. This study further emphasizes the role of M‐CSF, PTH, and 1,25‐(OH) 2 D 3 in osteoclastogenesis.