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Absence of paramyxovirus RNA in cultures of pagetic bone cells and in pagetic bon
Author(s) -
Birch M.A.,
Taylor W.,
Fraser W.D.,
Ralston S.H.,
Hart C.A.,
Gallagher J.A.
Publication year - 1994
Publication title -
journal of bone and mineral research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.882
H-Index - 241
eISSN - 1523-4681
pISSN - 0884-0431
DOI - 10.1002/jbmr.5650090103
Subject(s) - measles virus , morbillivirus , paramyxoviridae , canine distemper , virology , virus , paget's disease of bone , biology , polymerase chain reaction , nucleic acid , viral disease , pathology , measles , disease , medicine , gene , genetics , vaccination
It has been proposed that Paget's disease of bone is caused by the infection of bone cells with one or several paramyxoviruses. In this study we used the polymerase chain reaction (PCR), which allows the detection of very low levels of a target nucleic acid sequence, to study cultures of pagetic bone cells and samples of pagetic bone. Oligonucleotide primers were designed to flank a sequence of the nucleocapsid genome of measles virus and canine distemper virus (CDV). Within this fragment there were contrasting restriction endonuclease sites specific to measles or CDV that allowed identification of the original template. We were unable to detect paramyxovirus RNA in four strains of human bone cells outgrown from pagetic bone and one strain derived from an uninvolved site of a patient with Paget's disease. Furthermore, paramyxovirus sequences were not detected in cDNA prepared from six samples of pagetic bone biopsies. The work presented here further questions the role of measles and CDV in the abnormal remodeling observed in Paget's disease.