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1,25‐dihydroxyvitamin D 3 potentiates fluoride‐stimulated collagen type I production in cultures of human bone marrow stromal osteoblast‐like cells
Author(s) -
Kassem Moustapha,
Mosekilde Leif,
Eriksen Erik Fink
Publication year - 1993
Publication title -
journal of bone and mineral research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.882
H-Index - 241
eISSN - 1523-4681
pISSN - 0884-0431
DOI - 10.1002/jbmr.5650081207
Subject(s) - stromal cell , osteoblast , bone marrow , chemistry , human bone , calcitriol , endocrinology , fluoride , type i collagen , medicine , microbiology and biotechnology , biology , in vitro , vitamin d and neurology , biochemistry , inorganic chemistry
In this study we tested the effects of sodium fluoride (NaF) in serum‐free cultures of human marrow stromal osteoblast‐like [hMS(OB)] cells. NaF (10 −5 M) stimulated hMS(OB) cell proliferation up to 220% of control cultures. NaF alone did not increase type I collagen production, but in the presence of 1,25‐dihydroxyvitamin D 3 [1,25‐(OH) 2 D 3 ] (10 −9 M), NaF enhanced type I collagen production in a dose‐dependent way to 300% of 1,25‐(OH) 2 D 3 ‐treated control cultures. The production of alkaline phosphatase (ALP) and osteocalcin (bone gla protein, BGP) was also enhanced in the presence of 1,25‐(OH) 2 D 3 to 170 and 200%, respectively, of 1,25‐(OH) 2 D 3 ‐treated controls. Our results suggest that 1,25‐(OH) 2 D 3 potentiates fluoride‐mediated anabolism in hMS(OB) cell cultures and suggest that osteoblast precursors in bone marrow are targets for fluoride action.