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Immunoassay for urinary pyridinoline: The new marker of bone resorption
Author(s) -
Seyedin Saeid M.,
Kung Viola T.,
Daniloff Yuri N.,
Hesley Robert P.,
Gomez Baltazar,
Nielsen Lori A.,
Rosen Harold N.,
Zuk Robert F.
Publication year - 1993
Publication title -
journal of bone and mineral research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.882
H-Index - 241
eISSN - 1523-4681
pISSN - 0884-0431
DOI - 10.1002/jbmr.5650080515
Subject(s) - pyridinoline , deoxypyridinoline , immunoassay , bone resorption , urine , chemistry , chromatography , medicine , high performance liquid chromatography , immunogen , metabolic bone disease , endocrinology , antibody , immunology , biochemistry , osteoporosis , osteocalcin , enzyme , monoclonal antibody , alkaline phosphatase
Urinary pyridinoline (Pyd) and deoxypyridinoline (Dpd) are markers of bone resorption that are elevated above normal in subjects with metabolic bone disease. Total Pyd and Dpd, both free and peptide‐bound forms, can be measured by HPLC after hydrolysis and cellulose chromatography. Since free Pyd is the major component of total Pyd in urine, we developed an immunoassay using free Pyd as an immunogen. This assay is much easier to perform than HPLC, requires no sample preparation, and correlates well with total Pyd measurement by HPLC ( r = 0.97) and with urinary hydroxyproline ( r = 0.90). The antiserum reacts most strongly with free Pyd and Dpd and minimally with glycosylated and large peptide‐bound forms. The sensitivity of the Pyd immunoassay is less than 25 nM. The intraassay CV is 5–10%; the interassay CV is 10–15%. Analytic recovery studies indicated negligible sample interference. Furthermore, measurement of the Pyd in the same individuals over a 30 day time period exhibited minimal day‐to‐day variation. Thus, the Pyd immunoassay provides a rapid and easy method for evaluation of Pyd in urine. Pyd immunoassay may serve as a practical method of screening for metabolic bone disease and for monitoring therapeutic treatment.