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Autocrine regulators of MC3T3‐E1 cell proliferation
Author(s) -
Amarnani Saral,
Merriman Harold L.,
Linkhart Thomas A.,
Baylink David J.,
Mohan Subburaman
Publication year - 1993
Publication title -
journal of bone and mineral research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.882
H-Index - 241
eISSN - 1523-4681
pISSN - 0884-0431
DOI - 10.1002/jbmr.5650080206
Subject(s) - autocrine signalling , cell growth , growth factor , cell culture , microbiology and biotechnology , osteoblast , biology , insulin like growth factor , transforming growth factor beta , transforming growth factor , in vitro , receptor , biochemistry , genetics
MC3T3‐E1 cells, a clonal osteoblast‐like mouse calvarial cell line, secrete several growth regulating factors. These regulators include insulin‐like growth factor (IGF) type I, transforming growth factor β (TGF‐β), and IGF‐II in descending order of abundance. MC3T3‐E1 cells in culture also produce two IGF binding proteins (IGFBP), M r 25 and 32 kD, having sequence identity with IGFBP‐4 and IGFBP‐6, respectively. In addition, this is the first observation that osteoblast‐like bone cells in culture produce IGFBP‐6. To determine if growth factors produced by MC3T3‐E1 cells have autocrine actions on these cells, the effects of IGF‐I, IGF II, TGF‐β 1 , and IGFBP‐4 on MC3T3‐E1 cell proliferation were determined. Exogenous addition of IGF‐I and IGF‐II stimulated MC3T3‐E1 cell proliferation, but TGF‐β 1 , and IGFBP‐4 inhibited MC3T3‐E1 cell proliferation. Based on these findings, we conclude that MC3T3‐E1 cells in culture produce autocrine regulators of MC3T3‐E1 cell proliferation and that the actions of IGFs may also be regulated by IGFBPs produced by these same cells.