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A novel microcarrier bead model to investigate bone cell responses to mechanical compression in vitro
Author(s) -
Shelton Richard M.,
El Haj Alicia J.
Publication year - 1992
Publication title -
journal of bone and mineral research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.882
H-Index - 241
eISSN - 1523-4681
pISSN - 0884-0431
DOI - 10.1002/jbmr.5650071407
Subject(s) - microcarrier , bead , materials science , compression (physics) , strain (injury) , biomedical engineering , in vitro , displacement (psychology) , composite material , chemistry , cell , anatomy , biochemistry , biology , medicine , psychology , psychotherapist
Periosteal cells grown in macroporous microcarrier bead columns were cyclically loaded with compressive force using a newly developed model. In response to 1/2 h of cyclic compression, RNA synthesis increased significantly by twofold, from 113.4, 19.6 to 260.7 + 363 ( p lt; 0.01) after 16 h, whereas DNA synthesis did not increase significantly after 24 h. The microcarrier bead model was calibrated using a linear volume displacement transducer with a range of strain magnitudes applied across the column of 1000–6000 μstrain.