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Responsiveness to 1,25‐Dihydroxyvitamin D 3 is reduced in lymphocytes from osteoporotic women
Author(s) -
Koren R.,
Ravid A.,
Liberman U.A.,
Narinsky R.,
Maron L.,
Weisman M.,
Samuel R.
Publication year - 1992
Publication title -
journal of bone and mineral research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.882
H-Index - 241
eISSN - 1523-4681
pISSN - 0884-0431
DOI - 10.1002/jbmr.5650070908
Subject(s) - osteoporosis , endocrinology , medicine , calcitriol , peripheral blood mononuclear cell , vitamin d and neurology , ed50 , menopause , liter , thymidine , hormone , chemistry , in vitro , receptor , biochemistry
The purpose of this work was to test the hypothesis that reduced responsiveness of target organs to 1,25‐dihydroxyvitamin D 3 [1,25‐(OH) 2 D 3 ] is associated with osteoporosis. Peripheral blood mononuclear (PBM) cells have been previously shown to be a valid model for the action of 1,25‐(OH) 2 D 3 on its classic target organs in various pathologic and physiologic situations. The responsiveness of lymphocytes to the hormone can be assessed by the extent of inhibition it exerts on the proliferative response to mitogenic lectins. A group of 39 postmenopausal women, at least 10 years after the menopause, participated in the study. Osteoporosis, defined as the presence of at least one nontraumatic vertebral crush fracture, was diagnosed in 19 subjects. Mitogenesis of PBM cells stimulated by phytohemagglutinin and cultured for 72 h in the presence or absence of 1,25‐(OH) 2 D 3 (0.03–1 nmol/liter) was assessed by [ 3 H]thymidine incorporation during a 4 h pulse. The maximal inhibitory effect of 1,25‐(OH) 2 D 3 at saturating concentration (1 nM/liter) was 74.6 ± 2.8% (mean ± SEM) for normal compared to 65.3 ± 2.9% for osteoporotic women ( P = 0.015). The geometric mean of the ED 50 values of 1,25‐(OH) 2 D 3 was 60% higher in the osteoporotic than in the normal group ( P = 0.035). Our data are consistent with the notion that reduced responsiveness of target organs to 1,25‐(OH) 2 D 3 is associated with osteoporosis.