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Expression of insulin‐like growth factor I messenger ribonucleic acid in regenerating bone after fracture: Influence of indomethacin
Author(s) -
Edwall Dan,
Prisell P.T.,
Levinovitz A.,
Jennische E.,
Norstedt G.
Publication year - 1992
Publication title -
journal of bone and mineral research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.882
H-Index - 241
eISSN - 1523-4681
pISSN - 0884-0431
DOI - 10.1002/jbmr.5650070212
Subject(s) - endocrinology , medicine , insulin like growth factor , tibia fracture , in vivo , regeneration (biology) , growth factor , messenger rna , bone healing , tibia , callus , biology , receptor , anatomy , microbiology and biotechnology , biochemistry , genetics , gene
Expression of insulin‐like growth factor I (IGF‐I) was studied during time in the callus formed after tibial fracture in rats. Levels of IGF‐I mRNA in callus peaked on the day 8 postfracture, showing a 10‐ to 15‐fold induction compared to control bone. Levels of IGF‐I mRNA tended also to be increased in the fracture‐adjacent musculus tibialis anterior. IGF‐I immunoreactivity was found in cartilaginous cells, osteoblasts, and myocytes 6 and 8 days after fracture. No obvious differences were found between hypophysectomized animals and control animals with regard to IGF‐I immunoreactivity. Administration of the antiinflammatory drug indomethacin decreased the IGF‐I mRNA expression in the tibial fracture model. Previous findings have shown that IGF‐I is activated during in vivo muscle regeneration, and also in this model indomethacin administration reduces the expression of IGF‐I. The finding that indomethacin administration reduces IGF‐I expression could indicate that an inflammatory response may be important for activation of IGF‐I during tissue regeneration.