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Interaction of androgen and 1,25‐dihydroxyvitamin D 3 : Effects on normal rat bone cells
Author(s) -
Gray Colin,
Colston Kay W.,
Mackay Alan G.,
Taylor M. Louise,
Arnett Timothy R.
Publication year - 1992
Publication title -
journal of bone and mineral research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.882
H-Index - 241
eISSN - 1523-4681
pISSN - 0884-0431
DOI - 10.1002/jbmr.5650070107
Subject(s) - endocrinology , medicine , androgen , thymidine , stimulation , anabolism , dihydrotestosterone , testosterone (patch) , bone cell , inhibitory postsynaptic potential , biology , chemistry , in vitro , biochemistry , hormone
We studied the actions of testosterone (T) and 5α‐dihydrotestosterone (DHT) in combination with 1,25‐di‐hydroxyvitamin D 3 [1,25‐(OH) 2 D 3 ] on primary rat bone cells. The actions of androgens were generally anabolic, although response patterns varied considerably between cultures. For example, DHT caused striking dose‐ and time‐dependent increases in [ 3 H]thymidine incorporation into calvarial cells over the range 1–100 nM, with maximal stimulation of 2.5‐fold after 9 days in culture. Testosterone (50 nM) also stimulated [ 3 H]‐thymidine incorporation into long bone‐derived cells. 1,25‐(OH) 2 D 3 , generally blunted or abolished the proliferative action of androgens but was not itself always inhibitory; in some experiments, stimulation of [ 3 H]‐thymidine incorporation occurred. Collagen production, as assessed by [ 3 H]proline incorporation into pepsin‐resistant protein secreted by calvarial cells, was also stimulated by DHT. In some cultures, androgen responses were absent, although striking inhibitory responses to 1,25‐(OH) 2 D 3 were observed. These results illustrate complex patterns of responses to androgens and 1,25‐(OH) 2 D 3 in cells derived from rat bone.