Premium
Bone morphogenetic protein‐2b stimulation of growth and osteogenic phenotypes in rat osteoblast‐like cells: Comparison with TGF‐β 1
Author(s) -
Chen Theresa L.,
Bates Rebecca L.,
Dudley Andrew,
Hammonds Glenn R.,
Amento Edward P.
Publication year - 1991
Publication title -
journal of bone and mineral research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.882
H-Index - 241
eISSN - 1523-4681
pISSN - 0884-0431
DOI - 10.1002/jbmr.5650061216
Subject(s) - bone morphogenetic protein , osteoblast , alkaline phosphatase , bone morphogenetic protein 2 , transforming growth factor , bone morphogenetic protein 7 , microbiology and biotechnology , stimulation , chemistry , in vitro , in vivo , endocrinology , transforming growth factor beta , bone morphogenetic protein 6 , bone cell , medicine , biology , enzyme , biochemistry , genetics , gene
Abstract The biologic effects of recombinant human bone morphogenetic protein‐2b (BMP‐2b = BMP‐4) were studied and compared with transforming growth factor‐β 1 (TGF‐β 1 ) in fetal rat osteoblast‐like (ROB) cells. Similar to the effects of TGF‐β 1 , BMP‐2b stimulated DNA and collagen synthesis as well as protein accumulation. Unlike TGF‐β 1 , which inhibited alkaline phosphatase activity, BMP‐2b enhanced enzyme activity eight‐to ninefold over the control level. The present study demonstrates direct actions of BMP‐2b on bone‐associated cells to stimulate osteogenic phenotypes in vitro and provides a cellular mechanism for the induction of bone formation by BMP‐2b in vivo.