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Actions of calcipotriol (MC 903), a novel vitamin D 3 analog, on human bone‐derived cells: Comparison with 1,25‐dihydroxyvitamin D 3
Author(s) -
Evans Dean B.,
Thavarajah Mannicam,
Binderup Lise,
Kanis John A.
Publication year - 1991
Publication title -
journal of bone and mineral research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.882
H-Index - 241
eISSN - 1523-4681
pISSN - 0884-0431
DOI - 10.1002/jbmr.5650061207
Subject(s) - calcipotriol , osteocalcin , alkaline phosphatase , osteoblast , calcitriol , endocrinology , medicine , in vitro , vitamin d and neurology , vitamin , stimulation , prostaglandin e2 , chemistry , cell culture , biology , biochemistry , enzyme , immunology , genetics , psoriasis
Abstract The actions of a novel vitamin D 3 analog calcipotriol (MC 903), on human bone‐derived cells were compared to those of 1,25‐dihydroxyvitamin D 3 [1,25‐(OH) 2 D 3 ]. Both calcipotriol and 1,25‐(OH) 2 D 3 inhibited the proliferation of human osteoblast‐like cells in a dose‐dependent manner (10 −10 ‐10 −6 M), an effect observed at different cell densities. Lower concentrations of either agent exerted no marked effect on the growth of the cells compared to untreated cultures. Calcipotriol and 1,25‐(OH) 2 D 3 were equipotent in stimulating the activity of alkaline phosphatase and the synthesis of osteocalcin in human osteoblast‐like cells. The stimulation of alkaline phosphatase activity and osteocalcin synthesis by both compounds was evident by 24 h and was increased progressively up to 96 h in a dose‐dependent manner over the concentration range of 10 −10 ‐10 −6 M. The increment in both proteins was dependent on cell density and was attenuated at higher cell densities. In contrast to these actions, neither calcipotriol nor 1,25‐(OH) 2 D 3 (10 −14 ‐10 −6 M) affected the synthesis of prostaglandin E 2 . These studies indicate that calcipotriol and 1,25‐(OH) 2 D 3 exhibit a similar spectrum of activity on human osteoblast‐like cells in vitro.