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Parathyroid hormone‐related protein inhibits stimulated uterine contraction in vitro
Author(s) -
Shew Ronald L.,
Yee John A.,
Kliewer Douglas B.,
Keflemariam Yodit J.,
McNeill Daniel L.
Publication year - 1991
Publication title -
journal of bone and mineral research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.882
H-Index - 241
eISSN - 1523-4681
pISSN - 0884-0431
DOI - 10.1002/jbmr.5650060909
Subject(s) - contraction (grammar) , endocrinology , acetylcholine , myometrium , medicine , parathyroid hormone related protein , chemistry , uterine contraction , in vitro , muscle contraction , uterine horns , parathyroid hormone , calcium , biology , uterus , biochemistry
The effect of parathyroid hormone‐related protein (PTHrP) fragments 1–34, 38–64, and 67–86 on acetylcho‐line‐stimulated rat uterine contraction was examined in vitro. In addition, the possibility that PTHrP‐(38–64) or (67–86) influenced relaxation caused by PTHrP‐(1–34) was also investigated. Contraction of uterine horns was stimulated with 10 −6 or 10 −5 M acetylcholine. PTHrP‐(1–34) reduced the magnitude of acetylcho‐line‐stimulated uterine contraction. This effect was dose related over a concentration range of 10 −9 –10 −6 M. Neither PTHrP‐(38–64) or PTHrP‐(67–86) at concentrations of 10 −8 –10 −6 M affected uterine contraction stimulated by 10 −6 M acetylcholine. These fragments did not affect the relaxation caused by 10 −7 M PTHrP‐(1–34). These results demonstrate that (1) PTHrP‐(1–34) at 10 −6 M influences contraction of the rat myometrium and (2) the muscle relaxant activity of PTHrP is associated with the first 34 N‐terminal amino acids.