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Role of prostaglandins in interleukin‐1‐induced bone resorption in mice in vitro
Author(s) -
Akatsu Takuhiko,
Takahashi Naoyuki,
Udagawa Nobuyuki,
Imamura Kazunobu,
Yamaguchi Akira,
Sato Kanji,
Nagata Naokazu,
Suda Tatsuo
Publication year - 1991
Publication title -
journal of bone and mineral research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.882
H-Index - 241
eISSN - 1523-4681
pISSN - 0884-0431
DOI - 10.1002/jbmr.5650060212
Subject(s) - osteoclast , bone resorption , resorption , in vitro , prostaglandin e2 , cell culture , bone marrow , chemistry , endocrinology , spleen , medicine , microbiology and biotechnology , organ culture , biology , biochemistry , genetics
The mechanism of bone resorption induced by interleukin 1 (IL‐1) was examined in mice using three different in vitro assay systems: a fetal long bone organ culture system, a bone marrow culture system, and a co‐culture system of primary osteoblastic cell populations and spleen cells. In the organ culture system, recombinant human IL‐1α (rhIL‐1α) increased both bone resorption and osteoclast number. Both were partially suppressed in the presence of indomethacin. In the marrow culture, both rhIL‐1α and rhIL‐1β stimulated osteoclastlike cell formation, which was completely inhibited by adding indomethacin concurrently. Furthermore, there was a good correlation between the number of osteoclastlike cells formed and the amount of prostaglandin E 2 (PGE 2 ) released into the culture media. This indicates that PGE 2 is involved in the mechanism of IL‐1‐mediated osteoclastlike cell formation. In the coculture of primary osteoblastic cell populations and spleen cells, rhIL‐1 again stimulated osteoclastlike cell formation, which was inhibited by adding indomethacin. In the cocultures in which direct interaction between osteoblastic cells and spleen cells was inhibited, PGE 2 synthesis was similarly increased but no osteoclastlike cells were formed. These results indicate that IL‐1 induces osteoclast formation by a mechanism involving PG (most likely PGE 2 ). Furthermore, direct interaction between osteoclast progenitors and osteoblastic cells is required in the osteoclast recruitment induced by IL‐1.