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In vitro resorptive activity of isolated chick osteoclasts: Effects of carbonic anhydrase inhibition
Author(s) -
Hunter Susan J.,
Rosen Clifford J.,
Gay Carol V.
Publication year - 1991
Publication title -
journal of bone and mineral research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.882
H-Index - 241
eISSN - 1523-4681
pISSN - 0884-0431
DOI - 10.1002/jbmr.5650060111
Subject(s) - carbonic anhydrase , bone resorption , chemistry , resorption , in vitro , enzyme , sulfonamide , osteoclast , biochemistry , inhibitory postsynaptic potential , pharmacology , endocrinology , biology , stereochemistry
A potent inhibitor of carbonic anhydrase, 5‐[3‐hydroxybenzoyl]thiophene‐2‐sulfonamide (HTS), was shown to cause a 37% reduction in the area of resorption pits formed by isolated chick osteoclasts when used at a dose of 10 −7 M. HTS at doses of 10 −9 and 10 −7 M was also effective in reducing acid formation by the osteoclasts (14 and 36%, respectively). Additionally, the effect of HTS was found to be readily reversed by removing the agent, showing that it does not exert a toxic effect on the cells. This study indicates that the inhibitory effect of HTS on bone resorption is at the level of the acid‐forming mechanism in osteoclasts and supports the view that carbonic anhydrase has a central role in the process.