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Sodium fluoride lacks mitogenic activity for fetal human bone cells In Vitro
Author(s) -
Kopp Jeffrey B.,
Robey Pamela Gehron
Publication year - 1990
Publication title -
journal of bone and mineral research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.882
H-Index - 241
eISSN - 1523-4681
pISSN - 0884-0431
DOI - 10.1002/jbmr.5650051321
Subject(s) - calvaria , fluoride , sodium fluoride , endocrinology , medicine , osteoblast , bone cell , fetus , precursor cell , chemistry , in vitro , cell culture , fetal bovine serum , femur , biology , biochemistry , pregnancy , surgery , inorganic chemistry , genetics
Sodium fluoride has been shown to be effective therapy for some patients with vertebral osteoporosis. Data from histomorphometric studies in patients and animals suggest that at least part of this effect may be a consequence of a proliferative effect of fluoride, either direct or indirect, on the osteoblast or on an osteoblastic precursor cell. Experiments with osteoblastic cells derived from embryonic chick calvaria have demonstrated a mitogenic effect of fluoride. The present study examined whether fluoride affects in a similar way fetal human bone cells derived from femur or calvaria. Under a variety of culture conditions, including medium supplemented with serum and in serum‐free medium, fluoride did not alter the proliferative rate of human bone cells as measured by thymidine incorporation and direct cell counting.