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Effects of (3‐amino‐1‐hydroxypropylidene)‐1,1‐bisphosphonate on mouse osteoclasts
Author(s) -
Marshall Michael John,
Wilson Andrew Sean,
Davie Michael William John
Publication year - 1990
Publication title -
journal of bone and mineral research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.882
H-Index - 241
eISSN - 1523-4681
pISSN - 0884-0431
DOI - 10.1002/jbmr.5650050909
Subject(s) - osteoclast , endocrinology , bisphosphonate , medicine , calcitonin , parathyroid hormone , chemistry , in vitro , osteoporosis , calcium , biochemistry , receptor
A group of 5‐day‐old mice were injected intraperitoneally with (3‐amino‐1‐hydroxypropylidine)‐1,1‐bisphosphonate (APD). Morphologic changes were observed in vitally stained osteoclasts on parietal bones 3 days later, and these were judged to be degenerative. At this time significantly increased numbers of nuclei per osteoclast and total numbers of osteoclast nuclei were observed. However, at 4 days after the injection of APD, the total numbers of osteoclasts were significantly reduced relative to controls. When parietal bones were maintained in culture, APD reduced osteoclast numbers and inhibited cell‐mediated 45 Ca 2+ release. Exposure of bones to parathyroid hormone increased the number of osteoclasts counted 1 day later. This effect was not blocked by APD. Calcitonin prevented the reduction in osteoclast numbers due to APD in vitro. We conclude that APD has a direct effect on resorbing mouse osteoclasts