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Osteoclasts isolated from membranous bone in children exhibit nuclear estrogen and progesterone receptors
Author(s) -
Pensler Jay M.,
Radosevich James A.,
Higbee Russell,
Langman Craig B.
Publication year - 1990
Publication title -
journal of bone and mineral research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.882
H-Index - 241
eISSN - 1523-4681
pISSN - 0884-0431
DOI - 10.1002/jbmr.5650050802
Subject(s) - endocrinology , medicine , bone resorption , estrogen , estrogen receptor , chemistry , bone remodeling , receptor , calcitonin , biology , cancer , breast cancer
Osteoclasts were isolated from membranous bone from four children without metabolic bone disease who were undergoing craniotomy for either tumor or trauma. Both freshly isolated osteoclasts and those cultured for 4–7 days exhibited the following characteristics: production of tartrate‐resistant acid phosphatase (9.5–14.8 units), contraction in response to application of 100 mg/ml of human calcitonin, and formation of resorption lacunae on devitalized bone wafers. Nuclear estrogen and progesterone receptors were demonstrated by immunohistochemical techniques and quantitated in two of the patients by radioimmunoassay (estrogen receptor RIA, 23.6 and 23.8 cpm/μg protein; progesterone receptor RIA, 36.7 and 74.2 cpm/μg protein). The demonstration of sex steroid hormone receptors in the nucleus of osteoclasts derived from children with normal membranous bone has estabished a potential mechanism whereby direct modulation of bone resorption by the sex steroid estrogen and progesterone may occur.