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Regulation by calcium and 1,25‐(OH) 2 D 3 of cell proliferation and function of bovine parathyroid cells in culture
Author(s) -
Ishimi Yoshiko,
Russell John,
Sherwood Louis M.
Publication year - 1990
Publication title -
journal of bone and mineral research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.882
H-Index - 241
eISSN - 1523-4681
pISSN - 0884-0431
DOI - 10.1002/jbmr.5650050712
Subject(s) - calcium , endocrinology , parathyroid chief cell , medicine , incubation , cell culture , cell growth , secretion , parathyroid hormone , stimulation , biology , calcium metabolism , chemistry , microbiology and biotechnology , biochemistry , genetics
The effects of high calcium and 1,25‐(OH) 2 D 3 on parathyroid cell growth, PTH secretion, and steady‐state levels of pre‐proPTH mRNA in proliferating bovine parathyroid cells were examined. Cells were established in primary tissue culture and then tested in passages 2 and 5. Cell proliferation was suppressed by 10 −9 ‐10 −7 M 1,25‐(OH) 2 D 3 but not by high calcium (2.5 mM). Cells at passages 2 and 5 were grown to subconfluence and then exposed for 72 h to 2.5 mM calcium or 10 −7 M 1,25‐(OH) 2 D 3 . Pre‐proPTH mRNA was decreased to approximately 50% of control by 2.5 mM calcium compared with 0.3 and 1.0 mM calcium. PTH secretion, as tested by low calcium stimulation for 1 h at the end of 72 h incubation, was inhibited by 50% in cells that had been exposed to high calcium compared with control. Incubation with 10 −7 M 1,25‐(OH) 2 D 3 caused a decrease in the levels of pre‐proPTH mRNA and PTH release to 50% of control at 72 h. These results suggest that cultured bovine parathyroid cells, at least in early passages, have responses to high calcium and l,25‐(OH) 2 D 3 similar to those in primary nonproliferating cultures studied earlier and that 1,25‐(OH) 2 D 3 inhibits the proliferation of parathyroid cells in a dose‐responsive fashion.