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Effects of parathyroid hormone, calcitonin, and dibutyryl‐cyclic AMP on osteoclast area in cultured chick tibia
Author(s) -
Pandalai Sudha,
Gay Carol V.
Publication year - 1990
Publication title -
journal of bone and mineral research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.882
H-Index - 241
eISSN - 1523-4681
pISSN - 0884-0431
DOI - 10.1002/jbmr.5650050706
Subject(s) - calcitonin , osteoclast , parathyroid hormone , endocrinology , medicine , acridine orange , chemistry , tibia , calcium , anatomy , biochemistry , apoptosis , receptor
Abstract Slices of osteoclast‐enriched endosteal surfaces of 3 week chick tibia were cultured for 1‐3 days. Osteoclasts on the bone surface were made visible by acridine orange fluorescence. Osteoclast area was measured by image analysis. Parathyroid hormone (PTH) caused osteoclasts to increase in area about 40%, and calcitonin (CT) caused a decrease in area also of about 40%. Subsequent addition of dibutyryl cyclic AMP to PTH‐ or to CT‐treated cells resulted in a further change of 40 and 30%, respectively. Application of the cyclic AMP analog alone had no effect. All responses were rapid, occurring in 2‐4 minutes.