Premium
Mechanisms underlying the stimulation of PTH release by GppNHp in permeabilized bovine parathyroid cells
Author(s) -
Leboff Meryl S.,
Oetting Marguerite,
Brown Edward M.
Publication year - 1990
Publication title -
journal of bone and mineral research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.882
H-Index - 241
eISSN - 1523-4681
pISSN - 0884-0431
DOI - 10.1002/jbmr.5650050704
Subject(s) - inositol , inositol phosphate , inositol trisphosphate , adenylate kinase , egta , calcium , biology , endocrinology , parathyroid hormone , stimulation , phospholipase c , medicine , chemistry , biochemistry , enzyme , receptor
We examined changes in cAMP and inositol phosphate metabolism to assess the contribution of the guanine nucleotide regulatory (G) protein(s) regulating adenylate cyclase and phospholipase C in mediating the stimulatory effects of GppNHp on PTH release from permeabilized bovine parathyroid cells. To examine the role of G s , the G protein stimulating adenylate cyclase, and cAMP on PTH release, permeabilized cells were incubated with either GppNHp or isoproterenol, and the effects of these agents on PTH release and cellular cAMP content were determined by RIA. To study the effects of GppNHp on inositol phosphate accumulation, permeabilized cells prelabeled with [ 3 H]inositol were exposed to GppNHp, and inositol phosphates were measured using ion‐exchange chromatography. These studies revealed that isoproterenol produced a dose‐dependent increment in cAMP content in permeabilized cells with no significant effect on PTH release. Conversely, GppNHp rapidly and markedly elevated PTH release with a smaller and delayed rise in cAMP content. GppNHp‐ also promoted a dose‐dependent increase in inositol monophosphate (IP), inositol bisphosphate (IP 2 ), and inositol trisphosphate (IP 3 ) accumulation, suggesting activation of phosphoinositide hydrolysis. Addition of dioctanoylglycerol, however, a synthetic diacylglycerol (DG) that activates protein kinase C, produced a much smaller increment in PTH release than GppNHp. Moreover, reducing the free calcium concentration to < 10 −9 M by adding 10 mM EGTA to the permeabilization medium dissociated the effects of GppNHp and DG on secretion, increasing GppNHp‐stimulated PTH release while reducing PTH secretion evoked by DG. In summary, (1) changes in cAMP content cannot account for the effects of GppNHp on PTH release; and (2) GppNHp increases the hydrolysis of phosphoinositides, but the addition of exogenous diacylglycerol does not mimic quantitatively the effects of GppNHp on hormone release. Thus, a G protein may be involved in the control of PTH release in permeabilized cells, perhaps by directly stimulating secretion at a locus distal to the regulation of cAMP or phosphoinositide metabolism.