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Recombinant murine interferon‐γ inhibits the fusion of mouse alveolar macrophages in vitro but stimulates the formation of osteoclastlike cells on implanted syngeneic bone particles in mice in vivo
Author(s) -
Vignery Agnès,
NivenFairchild Tracy,
Shepard Michael H.
Publication year - 1990
Publication title -
journal of bone and mineral research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.882
H-Index - 241
eISSN - 1523-4681
pISSN - 0884-0431
DOI - 10.1002/jbmr.5650050613
Subject(s) - in vivo , in vitro , macrophage , bone resorption , multinucleate , resorption , alveolar macrophage , tumor necrosis factor alpha , interferon gamma , chemistry , peripheral blood mononuclear cell , cell fusion , microbiology and biotechnology , immunology , biology , pathology , cell , medicine , endocrinology , biochemistry
Osteoclasts are multinucleated cells that originate from the fusion of mononuclear precursors and are responsible for bone resorption. Indirect evidence from in vitro studies suggests that IFN‐γ and TNF‐α inhibit and stimulate bone resorption, respectively, but contradictory results have emerged from the literature regarding the effects of IFN‐γ on macrophage multinucleation. Using highly sensitive model systems, the present work demonstrates that, in mice, rMuIFN‐γ inhibits the fusion of alveolar macrophages in vitro but augments the number of osteoclastlike cells on implanted syngeneic bone particles in vivo. Although rMuTNF‐α fails to stimulate macrophage multinucleation in either system, treatment of implanted animals with rMuIFN‐γ appears to limit the inflammatory reaction and favor tissue repair.