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An automatic method for bone histomorphometry: Assessment with reference to usual static and dynamic parameters
Author(s) -
Juvin Robert,
Phelip Xavier,
Camus Emmanuel,
Usson Yves
Publication year - 1990
Publication title -
journal of bone and mineral research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.882
H-Index - 241
eISSN - 1523-4681
pISSN - 0884-0431
DOI - 10.1002/jbmr.5650050306
Subject(s) - osteoid , bone histomorphometry , reproducibility , osteomalacia , fully automatic , computer science , apposition , spectrum analyzer , osteoporosis , nuclear medicine , biomedical engineering , algorithm , mathematics , medicine , trabecular bone , pathology , anatomy , statistics , mechanical engineering , telecommunications , engineering
To perform a fast and reproducible analysis in bone histomorphometry, we developed an automatic method for calculating static and dynamic parameters. A color automatic image analyzer (SAMBA 200) was used to obtain the usual parameters of bone histomorphometry: bone volume (Cn‐BV%TV), osteoid volume (Cn‐OV%BV), and osteoid surface (Cn‐OS%BS). A specialized algorithm was designed for calculation of the mineral apposition rate (MAR). Eroded surface (Cn‐ES%BS) was read in a semiautomatic mode using a cursor. To validate this program, we input 30 samples from patients with bone disease (20 osteoporosis, 6 renal osteodystrophy, 2 osteomalacia, and 2 hyperparathyroidism) using manual and automatic modes. The results obtained showed a highly significant correlation with the usual manual method for all parameters: OS/BS, r = 0.93; OV/BV, r = 0.98; MAR, r = 0.90. With the automatic method, larger values were found for osteoid parameters and MAR and lower values for BV/TV. There were no statistical differences for OV/BV and MAR when compared to the reference manual method. This study establishes that automatic measurements of osteoid parameters and MAR can be performed by a fast analyzer with as good reproducibility and accuracy as the manual method.