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Transfection of calcitonin gene regulatory elements into a cell culture model of the C cell
Author(s) -
Cote Gilbert J.,
Abruzzese Ronald V.,
Gagel Robert F.,
Lips CEES J. M.
Publication year - 1990
Publication title -
journal of bone and mineral research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.882
H-Index - 241
eISSN - 1523-4681
pISSN - 0884-0431
DOI - 10.1002/jbmr.5650050210
Subject(s) - transfection , calcitonin , enhancer , gene , regulatory sequence , cell culture , microbiology and biotechnology , reporter gene , calcitonin gene related peptide , gene expression , biology , regulation of gene expression , dna , chemistry , endocrinology , biochemistry , genetics , neuropeptide , receptor
Calcitonin gene expression in the TT cell line can be regulated by phorbol esters, cAMP, glucocorticoids, and 1,25‐dihydroxyvitamin D 3 . To further study the regulation of this gene we have sequenced 1460 bases 5′ to the start of calcitonin gene transcription. This DNA sequence contains cis consensus elements for both phorbol ester‐ and cAMP‐responsive elements. To study the role of these elements, calcitonin 5′ flanking DNA was coupled to the human growth hormone gene as a reporter and transiently transfected into TT cells, a human thyroid C cell line. Treatment of transfected TT cells stimulated a two‐ to fivefold increase in reported gene product expression, confirming the existence of functional cAMP‐ and phorbol ester‐dependent enhancers within the calcitonin 5′ flanking sequence