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Ultrastructural localization of tartrate‐resistant, purple acid phosphatase in rat osteoclasts by histochemistry and immunocytochemistry
Author(s) -
Clark Samuel A.,
Ambrose Wallace W.,
Anderson Thomas R.,
Terrell Regina S.,
Toverud Svein U.
Publication year - 1989
Publication title -
journal of bone and mineral research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.882
H-Index - 241
eISSN - 1523-4681
pISSN - 0884-0431
DOI - 10.1002/jbmr.5650040315
Subject(s) - acid phosphatase , immunocytochemistry , golgi apparatus , tartrate , biochemistry , extracellular , organelle , tartrate resistant acid phosphatase , chemistry , alkaline phosphatase , immunohistochemistry , antiserum , enzyme , biology , microbiology and biotechnology , antibody , endocrinology , cell , immunology
Abstract The intracellular localization of the tartrate‐resistant purple acid phosphatase in osteoclasts of developing rat bone has been determined immunocytochemically using an antiserum to the purified bone‐derived purple acid phosphatase. The localization of the immunoreactivity was compared with the results of enzyme histochemistry using p ‐nitrophenylphosphate as substrate and 10 mM tartrate. Both methods revealed the presence of the enzyme in numerous vesicles of various sizes up to 2–3 μm in diameter and in granules. There was no immunoreactivity in the Golgi apparatus, and tartrate completely inhibited the histochemical activity of this organelle. No consistent extracellular activity could be detected, nor was any reaction product observed at the ruffled border. The localization of the tartrate‐resistant purple acid phosphatase in osteoclasts is consistent with an intracellular function for this enzyme.