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Effects of calcitonin gene‐related peptide on cyclic AMP formation in chicken, rat, and mouse bone cells
Author(s) -
Michelangeli V. P.,
Fletcher A. E.,
Allan E. H.,
Nicholson G. C.,
Martin T. J.
Publication year - 1989
Publication title -
journal of bone and mineral research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.882
H-Index - 241
eISSN - 1523-4681
pISSN - 0884-0431
DOI - 10.1002/jbmr.5650040220
Subject(s) - calcitonin gene related peptide , calcitonin , calvaria , endocrinology , medicine , parathyroid hormone , osteoblast , collagenase , chemistry , bone cell , biology , neuropeptide , calcium , receptor , biochemistry , in vitro , enzyme
Mixed bone cell cultures obtained by sequential collagenase‐trypsin digestion of newborn chick, rat, and mouse calvaria responded to calcitonin gene‐related peptide (CGRP) with a dose‐dependent increase in cyclic AMP formation. The amplitude of response to CGRP in each species was less than that to parathyroid hormone (PTH). The CGRP effect was not the result of an action as a weak calcitonin agonist, since in most instances a calcitonin effect was not observed. Only in early digests of mouse calvarial cells were consistent stimulatory effects of calcitonin on cyclic AMP noted, and these were always considerably less in amplitude than those to CGRP. It is concluded that chick, rat, and mouse bones contain cells in osteoblast‐rich populations that respond specifically to CGRP with a rise in cyclic AMP.