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Plasma carriers influence the uptake of cholecalciferol by human hepatoma‐derived cells
Author(s) -
Haddad John G.,
Aden David P.,
Aw T. Choon
Publication year - 1989
Publication title -
journal of bone and mineral research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.882
H-Index - 241
eISSN - 1523-4681
pISSN - 0884-0431
DOI - 10.1002/jbmr.5650040217
Subject(s) - cholecalciferol , lipoprotein , hep g2 , vitamin d and neurology , sterol , endocrinology , medicine , cell culture , vitamin d binding protein , in vivo , biology , albumin , chemistry , in vitro , biochemistry , cholesterol , genetics , microbiology and biotechnology
The uptake of [ 3 H]cholecalciferol by the human hepatoma‐derived cell lines Hep G2 and Hep 3B was examined as a function of the sterol's presentation on various plasma proteins at their native concentrations. Control cultures utilized devitalized cells cross‐linked with glutaraldehyde and estimated nonspecific sterol adherence to cells. With both cell lines, neither albumin nor plasma vitamin D binding protein permitted cholecalciferol uptake above control values. With Hep G2 cells, only low‐density lipoprotein presentation of the sterol resulted in significant cellular uptake that had features resembling a receptor‐mediated process. With Hep 3B, only high‐density lipoprotein presentation of the sterol resulted in a significant uptake that was cell, carrier, and time dependent. These results support the hypothesis that lipoprotein carriers could account for the efficient hepatic accumulation of cholecalciferol in vivo.