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A direct effect of 24,25‐(OH) 2 D 3 and 1,25‐(OH) 2 D 3 on the modeling of fetal mice long bones in vitro
Author(s) -
Schwartz Z.,
Soskolne W. A.,
Atkin I.,
Goldstein M.,
Ornoy A.
Publication year - 1989
Publication title -
journal of bone and mineral research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.882
H-Index - 241
eISSN - 1523-4681
pISSN - 0884-0431
DOI - 10.1002/jbmr.5650040205
Subject(s) - hydroxyproline , calcium , endocrinology , medicine , chemistry , resorption , metabolite , mineralization (soil science) , bone resorption , phosphorus , fetus , long bone , anatomy , biology , nitrogen , pregnancy , organic chemistry , genetics
To examine the effects of 24,25‐(OH) 2 D 3 and 1,25‐(OH) 2 D 3 on fetal long bone modeling the radii and ulnae of 16 day fetal mice were grown in vitro for 2 days. Their growth, mineralization, and resorption were assessed by measuring diaphyseal length, calcium and phosphorus content, hydroxyproline‐protein ratios, and the release of incorporated 45 Ca. The results showed that 24,25‐(OH) 2 D 3 at concentrations of 10 −10 ‐10 −8 M stimulated the growth of the bones as indicated by their increased diaphyseal length, periosteal bone area, and hydroxyproline content. Calcium and phosphorus content was significantly increased; 45 Ca release was unaltered. Bones incubated in media containing 10 −6 M 24,25‐(OH) 2 D 3 responded in a similar fashion to bones incubated in media containing 10 −10 ‐10 −8 M 1,25‐(OH) 2 D 3 , with inhibition of bone growth as indicated by reduced diaphyseal length, periosteal bone area, hydroxyproline‐protein ratios, and calcium and phosphorus content; 45 Ca release was significantly increased. Neither metabolite affected total bone length. The results suggest a role for 24,25‐(OH) 2 D 3 in the growth of fetal mice bones in vitro and also confirm the findings from previous studies that 1,25‐(OH) 2 D 3 and high concentrations of 24,25‐(OH) 2 D 3 stimulate bone resorption.