Premium
The effects of phenytoin on calcium uptake in osteoblastic cells
Author(s) -
Dziak R.,
Vernillo A.,
Rifkin B.
Publication year - 1988
Publication title -
journal of bone and mineral research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.882
H-Index - 241
eISSN - 1523-4681
pISSN - 0884-0431
DOI - 10.1002/jbmr.5650030408
Subject(s) - parathyroid hormone , calcium , endocrinology , phenytoin , medicine , chemistry , verapamil , cell culture , mechanism of action , bone cell , basal (medicine) , calcium metabolism , in vitro , biology , biochemistry , genetics , insulin , epilepsy , neuroscience
The effects of Phenytoin (diphenylhydantoin, DPH) on calcium uptake in osteoblastic cells were studied to elucidate the potential mechanism of action of this antiepileptic drug on bone metabolism. Preincubation of the human osteoblastic osteosarcoma) cell line, SaOS‐2, and normal rat osteoblastic cells with DPH decreased basal calcium uptake. This inhibition occurred at DPH doses from 0.1 to 50 μg/ml. Parathyroid hormone (PTH) and prostaglandin E 2 (PGE 2 ) increased calcium uptake in the SaOS‐2 cell line. Following preincubation with DPH, calcium uptake in cells treated with PTH or PGE 2 did not exceed control levels. However, significant increases in the PTH‐ or PGE 2 ‐treated + DPH‐pretreated cells compared to DPH pretreatment alone were still observed. These studies indicate that DPH induces decreases in osteoblastic calcium influx and they add further information on the possible mode of action of this drug on bone.