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Quantitative bone histomorphometry in nude mice bearing a human squamous cell lung cancer
Author(s) -
Kukreja Subhash C.,
Rosol Thomas J.,
Shevrin Daniel H.,
York Patricia A.
Publication year - 1988
Publication title -
journal of bone and mineral research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.882
H-Index - 241
eISSN - 1523-4681
pISSN - 0884-0431
DOI - 10.1002/jbmr.5650030314
Subject(s) - bone resorption , parathyroid hormone , endocrinology , medicine , bone remodeling , resorption , cancer , calcium
We performed quantitative bone histomorphometry on lumbar vertebrae in hypercalcemic tumor‐bearing athymic mice carrying a human squamous cell carcinoma. For comparison, studies were also performed in athymic mice that received bovine 1–34 parathyroid hormone (PTH) infusion at the rate of 0.167 μg/hr for 7 days. In both the PTH‐infused and tumor‐bearing animals, percent cortical and total bone areas were significantly reduced as compared to controls, whereas trabecular bone was significantly reduced only in the tumor‐bearing animals. Trabecular perimeter lined by osteoclasts was significantly increased in both tumor‐bearing (1.7‐fold) and PTH‐infused animals (2.8‐fold) compared to control mice. Trabecular perimeter lined by active osteoblasts was significantly reduced in the tumor‐bearing animals (to 42% of control) and unchanged in the PTH‐infused animals (97% of control). Tumor‐bearing animals had significantly reduced resorptive as well as formative surfaces as compared to the PTH‐infused animals. Dynamic histomorphometry revealed a marked reduction in bone formation rate (23% of control) in the tumor‐bearing animals. The studies therefore demonstrate a marked inhibition of bone formation associated with increased bone resorption in this model of hypercalcemia of malignancy. These observations are similar to those seen in the human syndrome.